Imaging and modeling plant ovule growth and architecture

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Context: Collaborative project IRD Montpellier, France (D. Autran, D. Grimanelli) – University of Zürich, Switzerland (C. Baroux) within an ANR-SNF funded consortium including expert teams in computational modeling (C. Godin, Virtual plants, INRIA Montpellier; O.Hamant, A.Bouaoud, RDP, ENS Lyon, France).

Profile: The position is open to candidates holding either a Master degree or a PhD (27 to 36 months depending on education level and experience) with experience in genetics, microscopy and molecular biology in plant biology. The candidate has a strong interest for developmental biology, a genuine motivation for computational approaches applied for growth modeling and image processing and excellent team work spirit. The position is based in Montpellier with working periods in Zurich (PhD applicant: will join a 36 months bi-national (“cotutelle internationale”) PhD Program with 12 months in Zurich; Postdoc: 6-10 months internships in Zürich to be defined). Starting date: as soon as possible.

Proposé par: Daphné Autran
Email: daphne.autran@ird.fr
Website: http://www.diade-research.fr/en/pages/research-group/redg.html
Laboratoire/Institution: IRD – Institut de Recherche pour le Developpement / University of Zurich, Switzerland
Adresse: 911 av Agropolis, Montpellier, 34000, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/IMAGO-position-in-plant-reproductive-development.pdf

3-year post-doctoral position in super-resolution imaging of bacterial division – IBS, Grenoble, France

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Description of the offer:
Bacterial cell division, a fundamental biological process and a source of multiple therapeutic targets, results from the combination of membrane constriction with cell wall synthesis. Coordination of these processes within a large protein complex (the divisome) ensures cell integrity and cell shape maintenance. The identity, the role, the structure and the protein interaction network of many division proteins are known. However, we still do not understand how all these proteins assemble into the bacterial cell to form a functional macromolecular complex in charge of membrane constriction and cell wall synthesis. In particular, the dynamics and the regulation of the divisome assembly along the cell cycle remain obscure. We will address these questions in the human pathogen Streptococcus pneumoniae through a research program combining bacterial genetics and super-resolution imaging. We are searching for a motivated post-doctoral fellow to study the in vivo nano-architecture of division proteins using 2-color PALM/dSTORM and single-particle tracking PALM.
Under the responsibility of the project leader (C. Morlot) in the Pneumococcus group and in close collaboration with the biophysicists of the Pixel team (D. Bourgeois, V. Adam) at the Institute for Structural Biology (IBS), the post-doc will:
– Grow and prepare S. pneumoniae cells for PALM/dSTORM and sptPALM studies
– Acquire, process and analyze 1- and 2-color PALM/dSTORM and sptPALM data
Requirements:
– Strong Ph.D. or post-doctoral experience in single-molecule localization microscopy (experience in 2-color PALM/dSTORM will be a plus)
– Autonomy, enthusiasm, excellent written and oral communication skills in english
Starting date: the position is open from May 2017, and will remain so until filled
Salary: depending on the experience of the candidate
Laboratory and environment:
Pneumococcus group – Institut de Biologie Structurale (IBS) – UMR5075 (CNRS/CEA/UGA) 71, avenue des martyrs – CS 10090 – 38044 GRENOBLE CEDEX 9, France
http://www.ibs.fr/research/research-groups/pneumococcus-group-t-vernet/
Grenoble is situated in the middle of the beautiful French Alps, and provides a unique environment for state‐of‐the‐art integrated cellular and structural biology (http://www.psb‐ grenoble.eu/).
Contact:
Applications (CV, motivation letter and 2 reference letters) should be sent to Cécile Morlot Email: cecile.morlot@ibs.fr Phone: +33 (0)4 57 42 86 55

Proposé par: Cecile Morlot
Email: cecile.morlot@ibs.fr
Website: http://www.ibs.fr/research/research-groups/pneumococcus-group-t-vernet/
Laboratoire/Institution: Institut de Biologie Structurale
Adresse: 71, avenue des martyrs – CS 10090, Grenoble, 38044, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Post-doc-offer-DIVinHD.pdf

Open post-doctoral position Intravital correlative imaging of tumor metastasis

Type d’annonce: Proposition – PostDoc

Description de la proposition:

The Goetz lab (www.goetzlab.com) is looking for a motivated post-doctoral fellow interested in dissecting tumor metastasis using intravital correlative microscopy. The work will be performed in Strasbourg (France) under the supervision of Jacky G.Goetz, in collaboration with international experts of tumor metastasis and imaging.
Our team studies the molecular and biomechanical events underlying tumor invasion, angiogenesis and metastasis. To this end, we are using a combination of models ranging from in vitro cell biology and biophysical assays (Goetz et al., Cell, 2011) to in vivo animal models of tumor progression (mice and zebrafish) (Goetz et al., Cell Reports, 2014). In particular, we are developing intravital imaging and intravital CLEM (Correlated Light and Electron Microscopy) technologies for tracking subcellular events in vivo at high-resolution (Karreman et al. PloS One 2014; Karreman et al., Journal of Cell Science, 2016; Karreman et al., Trends in Cell Biology, 2016). This development is performed in close collaboration with Y.SCHWAB (EMBL, Heidelberg). More recently, our team was involved in the identification of a new molecular driver of exosome biogenesis (Hyenne et al., Journal of Cell Biology, 2015).
This project aims to dissect the subcellular mechanisms underlying tumor metastasis at very high-resolution using intravital correlative microscopy. Metastasis can be considered as the end product of a multistep process where cancer cells disseminate to distant organs and home in a new tissue microenvironment. Metastases are responsible for the large majority of cancer-related deaths. However, the molecular and cellular mechanisms driving metastasis formation remain to be elucidated and better described in a realistic in vivo context. In collaboration with the team of Y.Schwab (EMBL), we recently developed a technique called intravital correlative microscopy. Here, we propose to apply the newly-developed technology to increase our understanding of tumor metastasis, in particular in terms of cell protrusitivity, proteolytic activity, adaptability to local physical barriers and ability to communicate with its surrounding during the metastasis cascade.
The project involves collaboration with international teams (EMBL, DKFZ, Curie institute) and uses state-of-the-art imaging technologies and animal models. We are looking for an enthusiastic and motivated fellow (who recently defended his Ph.D), with a background in cell and cancer biology and imaging. Expertise in intravital imaging and electron microscopy will be very much appreciated.
Interested candidates should apply as soon as possible or before: 2016 October 1st

Contact:
Jacky G.Goetz, jacky.goetz@inserm.fr, INSERM U1109, Strasbourg, France
Web: www.goetzlab.com, Twitter: @GoetzJacky

Associated references:
1. Karreman, M. A. et al. Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points. PloS One 9, e114448 (2014).
2. Karreman, M. A. et al. Fast and precise targeting of single tumor cells in vivo by multimodal correlative microscopy. J. Cell Sci. 129, 444–456 (2016).
3. Follain, G., Mercier, L., Osmani, N., Harlepp, S. & Goetz, J. G. Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology. J. Cell Sci. (2016). doi:10.1242/jcs.189001
4. Karreman, M. A., Hyenne, V., Schwab, Y. & Goetz, J. G. Intravital Correlative Microscopy: Imaging Life at the Nanoscale. Trends Cell Biol. (2016). doi:10.1016/j.tcb.2016.07.003

Proposé par: JACKY GOETZ
Email: JACKY.GOETZ@INSERM.FR
Website: http://WWW.GOETZLAB.COM
Laboratoire/Institution: INSERM U1109
Adresse: 3, avenue Molière, Strasbourg, 67200, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Add_MetaCLEM_post-doc-september-16.pdf

4D imaging of the blood microcirculation by Digital Holographic Microscopy DHM

Type d’annonce: Proposition – PostDoc

Description de la proposition:

The post doc position is available for a period of one year in the soft material team of
laboratory Charles Coulomb in Montpellier. Supervision is provided by a physicist/ optician
(Michel Gross) and a biologist (Daniel Alexandre), members of this team.

Applicants must hold a PhD degree in the closely related area (physics, optics, or
biomedical engineering) prior to the start-date of the position. Applicants with PhD degrees in
electrical engineering with interdisciplinary experiences and complementary expertise in the
areas of optics, biophysics, microbiology are also highly encouraged to apply. Experiences and
expertises in following areas: optics, microscopy, numerical techniques in C/C++ and with GPU,
computational imaging, modern coherent optics and holography are also welcome.

Proposé par: Michel GROSS
Email: michel.gross@univ-montp2.fr
Website: http://www.coulomb.univ-montp2.fr/index.php?page=pageperso&nom=GROSS&prenom=Michel
Laboratoire/Institution: Laboratoire Charles Coulomb – UMR 5221 CNRS-UM2
Adresse: Université Montpellier II Bat 11 CC 26. Place Eugène Bataillon, Montpellier, 34095, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Post-doc.pdf

Post-doc : Systematic analysis of gene expression in single intact cells by high throughput detection of single mRNAs

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Characterizing gene expression at the level of single cells and in the context of the cellular space is a difficult challenge, but meeting it will certainly drive important conceptual breakthroughs. Single molecule FISH (smFISH) identifies and localizes all mRNA molecules produced by a given gene, in every cell of large populations. SmFISH thus possesses unique advantages to study mRNA localization at the sub-cellular level. This is a fundamental but understudied phenomenon, involved in many biological processes, including mitosis, cell polarity, co-translational assembly of protein complexes etc…
In this project, we aim at mixing traditional and innovative approaches to combine the power of traditional smFISH techniques with the systematic aspects of genome-wide approaches. We will collect high-resolution, high quality images of the localization of many mRNAs in native fixed cells. Our first goal will be to discriminate localized mRNA from randomly distributed ones. Our second goal will be to group localized mRNAs into functional categories, to understand the functions of this process at the cellular level. This project will be performed in collaboration with the image analysis group of C. Zimmer (Pasteur Institute).

Proposé par: Edouard BERTRAND
Email: edouard.bertrand@igmm.cnrs.fr
Website: http://www.igmm.cnrs.fr/spip.php?rubrique150
Laboratoire/Institution: Edouard Betrand Lab, IGMM – CNRS UMR 5535
Adresse: 1919 route de Mende, Montpellier, 34090, France
Details en pdf:

http://gdr-miv.fr/gdr/wp-content/uploads/formidable/AnnoncePostDocBetrandLab-2016.pdf