IMAGING DNA REPAIR AT SUPER RESOLUTION

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Laboratory: Nuclear dynamics, UMR 3664
Angela Taddei Team: Compartmentalization and dynamics of nuclear functions.
Starting date: as soon as possible.

SUMMARY OF LAB’S INTERESTS:
The eukaryotic genome is packaged into large-scale chromatin structures that occupy distinct domains in the nucleus. This DNA organization is a key contributor to genome functions. Our aim is to understand what determines the spatial and temporal behavior of chromatin and how this affects two essential functions of the genome: gene expression and the maintenance of genome integrity. To understand these fundamental processes, we combine genetics, molecular biology and advanced live cells imaging.

PROJECT:
In this project, we propose to investigate the molecular mechanisms of repair proteins inside cells using super-resolution microscopy (photo-activable localization microscopy PALM and single particle tracking) in Saccharomyces cerevisiae yeast. In response to double strand break (DSB), repair proteins such as Mre11, Rad52 and Rad51, colocalize from a diffuse distribution to a repair focus located at the damaged DNA site. An enduring question in the DNA damage field is how do repair proteins find the correct target and how these membrane-less compartments are maintained and dissolved.
To answer these questions, we use PALM/STORM and single particle tracking approaches to elucidate the internal structure of repair foci and the dynamics of individual repair proteins. More specifically, the internal structure gives access to the shape, size and stoichiometry of repair foci; the dynamics of single repair proteins gives access to diffusion coefficients, kon/koff, residence time inside a focus, exchange rates between proteins inside the focus and the rest of the nucleus… The precise nature of repair foci will be studied for different types of DNA damages (endonuclease, chemical damaging agents, ionizing-irradiations).
We have already started to investigate the mobility of Rad52 proteins, an essential protein of homologous recombination in Saccharomyces cerevisiae yeast. To better understand the molecular mechanisms of DNA repair, we will study the nature of repair foci in mutant Rad52 defective in their interactions with its partners, before and after induction of different types of DNA damage(s).
We offer a 12-months founded post-doc contract and will support the candidate for post-doc grants applications.

Key publications:
Multi-scale tracking reveals scale-dependent chromatin dynamics after DNA damage. Miné-Hattab et al., Mol Biol Cell. 2017.
Recombination at subtelomeres is regulated by physical distance, double strand break resection and chromatin status. Batté et al., EMBO 2017.
Increased chromosome mobility facilitates homology search during recombination. Miné-Hattab et al., Nat Cell Biol. 2012.

ENVIRONMENT:
The Taddei team provides all equipment’s necessary for yeast cells cultures. A super resolution microscope is available in the unit (3D multi colors PALM/STORM and single particle tracking routinely used, and possibilities to install a SIM). Many yeast strains have been already developed for PALM/STORM and single particle experiments. The UMR 3664 of the Curie Institute is a very stimulating environment to work in the epigenetics and DNA repair field. The project in carried out in collaboration with Maxime Dahan’s team (Physico-Chimie Curie, UMR168).

PROFILE AND EXPERTISE:
We invite applications from highly motivated and dynamics individuals holding, or shortly expecting to be awarded, a PhD degree in biophysics. Proven research skills (publication track) as well as good communication skills (in English) are essential.

Expertise:
– Microscopy, image analysis (ImageJ, Matlab)
– Strong interest in cell biology and DNA repair mechanisms
– Knowledge in DNA repair mechanisms is appreciated
– Organization, responsibility and autonomy are required
– An experience in budding yeast and cell culture is appreciated

To apply, please send a letter, your CV including publication record
and contact details for 2 referees at:
Angela.taddei@curie.fr, Judith.mine@curie.fr

Proposé par: judith Mine-Hattab
Email: judith.Mine@curie.fr
Website: https://science.curie.fr/recherche/developpement-cancer-genetique-epigenetique/umr-3664-dynamique-du-noyau/equipe-taddei/
Laboratoire/Institution: UMR3664 Nuclear dynamics, Taddei team / Institut Curie
Adresse: 26 rue d’ULM, Institut Curie Reserche center, Paris, 75005, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/6/Post_Doc_DNA_Repair_Super_Resolution_Microscopy-1.pdf

Frontiers of silencing at super resolution

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Laboratory: Nuclear dynamics, UMR 3664
Angela Taddei Team: Compartmentalization and dynamics of nuclear functions.
Starting date: as soon as possible

SUMMARY OF LAB’S INTERESTS:
The eukaryotic genome is packaged into large-scale chromatin structures that occupy distinct domains in the nucleus. This DNA organization is a key contributor to genome functions. Our aim is to understand what determines the spatial and temporal behavior of chromatin and how this affects two essential functions of the genome: gene expression and the maintenance of genome integrity. To understand these fundamental processes, we combine genetics, molecular biology and advanced live cells imaging.

PROJECT:
One of the most intriguing features of nuclear organization is the existence of sub-nuclear compartments enriched with specific DNA, RNA sequences and proteins. How such membrane-less micro-environments are formed and maintained remains unclear. Silent chromatin provides an evolutionary conserved example of such functional sub-compartments. In Saccharomyces cerevisiae yeast, the 32 telomeres of haploid cells cluster into 3-5 sub-nuclear compartments or telomere foci concentrating the SIR complex at the nuclear periphery. Our team has recently shown that this organization is dynamics and varies according to the metabolic status of the cell. In particular, cells group their telomeres into a unique cluster (hypercluster) upon progressive carbon source exhaustion, reshaping the genome architecture into a conformation that may help to maintain the longevity of quiescent cells.

In this project, we propose to combine yeast genetics with super resolution (PALM/STORM) and single particle tracking to decipher the biophysical mechanisms underlying telomere foci formation and dynamics.

We offer a 18-months founded post-doc contract and will support the candidate to apply for post-doc grants.

Key publications:
Multi-scale tracking reveals scale-dependent chromatin dynamics after DNA damage. Miné-Hattab et al., Mol Biol Cell. 2017.
Spatial reorganization of telomeres in long-lived quiescent cells. Guidi et al. Genome Biol. 2015.
Spatial telomere organization and clustering in yeast Saccharomyces cerevisias nucleus is generated by random dynamics of aggreagation-dissciation. Hozé et al., Mol. Biol. Cell. 2013.

ENVIRONMENT:
The Taddei team provides all equipment’s necessary for yeast cells culture and has a strong expertise in field of silencing and quiescence. A super resolution microscope is already available in the unit (PALM/STORM and single particle tracking in 3D with multi colors). Many yeast strains have been already developed for PALM/STORM and single particle experiments. The UMR 3664 of the Curie Institute is a very stimulating environment to work in the epigenetics and DNA repair field. The project is carried out in collaboration with Maxime Dahan’s team (Physico-Chimie Curie, UMR168).

PROFILE AND EXPERTISE:
We invite applications from highly motivated and dynamics individuals holding, or shortly expecting to be awarded, a PhD degree in biophysics. Proven research skills (publication track) as well as good communication skills (in English) are essential.

Expertise:
– Microscopy, image analysis (ImageJ, Matlab)
– Strong interest in cell biology and DNA repair mechanisms
– Knowledge in transcription and genetics is appreciated
– Organization, responsibility and autonomy are required
– An experience in budding yeast and cell culture is appreciated

To apply, please send a letter, your CV including publication record
and contact details for 2 referees at:
Angela.taddei@curie.fr, Judith.mine@curie.fr

Proposé par: Angela Taddei
Email: judith.Mine@curie.fr
Website: https://science.curie.fr/recherche/developpement-cancer-genetique-epigenetique/umr-3664-dynamique-du-noyau/equipe-taddei/
Laboratoire/Institution: UMR3664 Nuclear dynamics, Taddei team / Institut Curie
Adresse: 26 rue d’ULM, Institut Curie Reserche center, Paris, 75005, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/6/Post_Doc_Institut-Curie_Silencing_Super_Resolution_Microscopy-1.pdf

Polarbio: Polarimetric orthogonally breaking imaging applied to the study of the dynamics of intracellular architecture

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Interdisciplinary Post-doctoral position in optics and biology in Rennes

The objective of the Polarbio project is to integrate an innovative polarimetric imaging methodology on a conventional confocal microscope within the MRic imaging platform of the University of Rennes 1 in order to produce polarimetric contrast images on different types of biological samples. After a first proof of concept,
two research axes will be investigated: (i) the use of this contrast to characterize biological structures and (ii) the refinement of the microscopy method to improve acquisition speed and data analysis, such as wide field detection or new modes of polarization at the excitation or the emission side.

Research Laboratories: IGDR – R&D Microscopy team /Optics and Photonics dpt. (FOTON Institute)
Heads of the Scientific Project: Marc Tramier / Mehdi Alouini
Contacts: marc.tramier@univ-rennes1.fr / julien.fade@univ-rennes1.fr

Proposé par: Julien Fade
Email: julien.fade@univ-rennes1.fr

Laboratoire/Institution: Institut de Génétique et Développement de Rennes
Adresse: Campus Santé Villejean, RENNES, 35042, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/6/Post-doc-Offer-Polarbio-1.pdf

POSTDOCTORAL POSITION AVAILABLE IN MOLECULAR AND CELLULAR NEUROENDOCRINOLOGY

Type d’annonce: Proposition – PostDoc

Description de la proposition:

We are seeking a highly motivated postdoctoral fellow to study CgA/phosphatidic acid (PA) interaction at
the membrane level in living neuroendocrine cells. To do this, fluorescent molecular tools are available
and novel photoactivatable and biocompatible PA probes are currently synthesized. By combining cell
biology and biochemistry methods, state-of-the-art imaging methods in cellular and in vivo models, the
successful candidate will study CgA/PA interactions and the consecutive formation of microdomains at the
endomembrane level. Therefore, we will test the ability of our chemically synthesized PA analogues to
rescue membrane trafficking defects in neuroendocrine cells from transgenic animal models.
The candidate should have completed a PhD in biochemistry, molecular biology or cell biology. Experience
with membrane dynamics, fluorescence microscopy on live cells and image analysis is required. The
candidate will be working at Inserm 1239, in collaboration with CNRS UPR 3212 (Strasbourg), CNRS UMR
6014 (Rouen) research units and the CIML platform (Marseille) to take benefit of an interdisciplinary
environment (with biologists, biochemists, chemists and physical chemists) and of state-of-the-art
facilities for cell imaging (confocal, TIRF, gated-STED, electron, light sheet microscopies), fluorescence
correlation spectroscopy, flow cytometry, proteomics and animal housing.

Proposé par: Maité MONTERO
Email: maite.montero@univ-rouen.fr

Laboratoire/Institution: Inserm U1239
Adresse: Université de Rouen, Rouen, 76000, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/6/profil-Montero-post-doc-FRM.pdf

Post-doctoral position in Multiphoton and FLIM microscopy for metabolic imaging

Type d’annonce: Proposition – PostDoc

Description de la proposition:

The Advanced Microscopies & Tissue Physiology group at the Laboratory for Optics and Biosciences (LOB), Ecole Polytechnique, University of Paris-Saclay, France, is seeking a highly motivated and talented postdoctoral scientist with interest in interdisciplinary optical microscopy and biophysics research.

The Laboratory develops advanced methods in nonlinear optics, tissue microscopy, image analysis, cell/developmental biology, and biophysics for studying intact biological tissues with subcellular resolution.

Candidates should have a solid background and good experimental skills in optics and/or microscopy and should be strongly motivated and keen to work in an interdisciplinary research field. Previous experience in optical imaging and fluorescence lifetime microscopy will be an asset.

The successful candidate will develop novel approaches for fast fluorescence lifetime imaging of endogenous fluorophores in stem cells and living tissues with application to metabolic imaging (Stringari et. al PNAS 2011, Stringari et al Sci. Rep 2012, Aguilar-Arnal et. al PNAS 2016, Stringari et al Sci. Rep 2017).

The postdoctoral fellowship is funded by a 2017 Human Frontier Science Program Young Investigator collaborative grant. The awarded project: « Chromatin dynamics and nuclear metabolism: an intimate interplay uncovered by non-linear optics » aims to understand epigenetic mechanisms during stem cell differentiation and in particular investigate the influence of cellular metabolism on gene expression.

The postdoctoral appointment will be for 2 years. Detailed information about the research can be requested via email. Interested applicants should submit a CV to Chiara Stringari at chiara.stringari@polytechnique.edu before September 15th. Applications will be reviewed on a rolling basis, starting immediately.

Proposé par: Chiara Stringari
Email: chiara.stringari@polytechnique.edu
Website: https://portail.polytechnique.edu/lob/en/research/advanced-microscopies-tissue-physiology
Laboratoire/Institution: LABORATORY FOR OPTICS AND BIOSCIENCES , CNRS UMR7645 – INSERM U696
Adresse: Ecole Polytechnique Route de Saclay, Palaiseau, 91128, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/6/2017-07-Postdoc_FLIM_Stringari.pdf