Adaptive Optics for aberrating and scattering media

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Optical microscopy in biology develops today along two major directions: improving imaging quality, especially in terms of resolution and 3D field of view; and improving content and quantitativeness of associated measurements, such as molecular concentration and dynamics (diffusion, interactions, etc.). For obtaining the latter information, a very successful and well-established technique is Fluorescence Fluctuation Microscopy (FFM), which is a generalisation of classical Fluorescence Correlation Spectroscopy (FCS), which provides quantitative measurements of molecular concentration and mobility in living specimen, by analyzing the signal fluctuations caused by fluorescent molecules as they diffuse across a small observation volume. Within this context, the main goals of the μ-SCATTAB project are: i) to understand how light scattering and optical aberrations disturb measurements performed with Fluorescence Fluctuation Microscopy; ii) to develop new schemes to correct for these effects; iii) or alternatively, to take benefit of light scattering to perform molecular measurements.
In the past, Adaptive Optics (AO) approaches have been successfully used in microscopy for correcting sample-induced aberrations, but the majority of these methods have been concerned with compensating low-order aberrations which arise in rather transparent samples or at shallow focusing depth. However, when focusing deep into a specimen (tens to hundreds of μm), large-amplitude aberrations with complex phase structure arise, and light scattering dramatically increases. In our project, we will investigate how to take these effects into account and what are the most efficient correction schemes and strategies for FFM. Complementary experiments and numerical / theoretical calculations will be conducted by the Grenoble and Göttingen partners, which will imply visits between the two groups. We are looking of a post-doctoral researcher to join the Grenoble group: the contract should begin in September or October 2017 for a total duration up to 30 months.
The successful candidate would have a background in optics with strong experience in instrumental optics/microscopy. Previous experience in wavefront manipulation, adaptive optics or imaging through scattering media would also be highly appreciated. The recruited post-doc will join an interdisciplinary team of physicists and biophysicists working on living systems at the single cell or multicellular scale. He/she would be in charge of modifying a currently existing adaptive confocal microscope available in Grenoble to add a wavefront corrector with a large number of degrees of freedom and developing new strategies to perform FFM measurements in complex media.

Proposé par: Antoine DELON
Email: antoine.delon@univ-grenoble-alpes.fr
Website: http://www-liphy.ujf-grenoble.fr/Equipe-MOTIV
Laboratoire/Institution: laboratoire Interdisciplinaire de Physique (LIPhy)
Adresse: Université Grenoble Alpes Pôle Phitem Laboratoire LIPhy CS 40 700, GRENOBLE CEDEX 9, 38058, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/µ-scattab-position-final.pdf

Postdoctoral Position – Zebrafish models of proteinopathies in neurodegenerative diseases– Institut de Recherches Servier

Type d’annonce: Proposition – PostDoc

Description de la proposition:

The Servier’s Neuropsychiatry Innovation Therapeutic Unit accommodates a zebrafish platform as a part of its experimental research, located near Paris, in Croissy-sur-Seine, France. The platform is involved in early drug development and is strongly oriented towards providing tools to screen compounds intended for neurodegenerative diseases. The zebrafish team is also involved in the project IMPRiND, which is a public-private partnership, supported by the Innovative Medicines Initiative (IMI) and will officially start on Q2 2017. The project’s goal is to identify disease-relevant misfolded assemblies of alpha-synuclein and tau proteins and validate complex cellular systems and improve existing animal models of Alzheimer’s and Parkinson’s disease.
We are looking for a Postdoctoral research Associate to contribute to the development and characterization of zebrafish models for alpha-synuclein and tau propagation in conjunction with the project IMPRiND as well as our internal research activities. The ideal candidate will have a PhD in biological sciences or related disciplines, with a previous experience in the field of proteinopathies, strong knowledge of immunostaining, fluorescence microscopy and image analyses, as well as a strong interest in using zebrafish as a tool for drug discovery and target validation in neurodegenerative diseases. Good communication skills in English and French are a must to interact with the partners in the European consortium IMPRiND and the multi-disciplinary team at our Research Center, respectively.
Servier is an independent research-based pharmaceutical company headquartered in France. With a strong international presence in 148 countries and 92% of its medicines being prescribed outside of France, Servier employs more than 21,200 people worldwide. In 2015, the company recorded a turnover of 3.9 billion euros of which 24% was reinvested in research and development.

Proposé par: Anne Dekeyne
Email: anne.dekeyne@servier.com

Laboratoire/Institution: Institut de Recherches SERVIER
Adresse: 125 chemin de ronde, Croissy sur Seine, 78720, France
Details en pdf:

Imaging and modeling plant ovule growth and architecture

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Context: Collaborative project IRD Montpellier, France (D. Autran, D. Grimanelli) – University of Zürich, Switzerland (C. Baroux) within an ANR-SNF funded consortium including expert teams in computational modeling (C. Godin, Virtual plants, INRIA Montpellier; O.Hamant, A.Bouaoud, RDP, ENS Lyon, France).

Profile: The position is open to candidates holding either a Master degree or a PhD (27 to 36 months depending on education level and experience) with experience in genetics, microscopy and molecular biology in plant biology. The candidate has a strong interest for developmental biology, a genuine motivation for computational approaches applied for growth modeling and image processing and excellent team work spirit. The position is based in Montpellier with working periods in Zurich (PhD applicant: will join a 36 months bi-national (“cotutelle internationale”) PhD Program with 12 months in Zurich; Postdoc: 6-10 months internships in Zürich to be defined). Starting date: as soon as possible.

Proposé par: Daphné Autran
Email: daphne.autran@ird.fr
Website: http://www.diade-research.fr/en/pages/research-group/redg.html
Laboratoire/Institution: IRD – Institut de Recherche pour le Developpement / University of Zurich, Switzerland
Adresse: 911 av Agropolis, Montpellier, 34000, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/IMAGO-position-in-plant-reproductive-development.pdf

3-year post-doctoral position in super-resolution imaging of bacterial division – IBS, Grenoble, France

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Description of the offer:
Bacterial cell division, a fundamental biological process and a source of multiple therapeutic targets, results from the combination of membrane constriction with cell wall synthesis. Coordination of these processes within a large protein complex (the divisome) ensures cell integrity and cell shape maintenance. The identity, the role, the structure and the protein interaction network of many division proteins are known. However, we still do not understand how all these proteins assemble into the bacterial cell to form a functional macromolecular complex in charge of membrane constriction and cell wall synthesis. In particular, the dynamics and the regulation of the divisome assembly along the cell cycle remain obscure. We will address these questions in the human pathogen Streptococcus pneumoniae through a research program combining bacterial genetics and super-resolution imaging. We are searching for a motivated post-doctoral fellow to study the in vivo nano-architecture of division proteins using 2-color PALM/dSTORM and single-particle tracking PALM.
Under the responsibility of the project leader (C. Morlot) in the Pneumococcus group and in close collaboration with the biophysicists of the Pixel team (D. Bourgeois, V. Adam) at the Institute for Structural Biology (IBS), the post-doc will:
– Grow and prepare S. pneumoniae cells for PALM/dSTORM and sptPALM studies
– Acquire, process and analyze 1- and 2-color PALM/dSTORM and sptPALM data
Requirements:
– Strong Ph.D. or post-doctoral experience in single-molecule localization microscopy (experience in 2-color PALM/dSTORM will be a plus)
– Autonomy, enthusiasm, excellent written and oral communication skills in english
Starting date: the position is open from May 2017, and will remain so until filled
Salary: depending on the experience of the candidate
Laboratory and environment:
Pneumococcus group – Institut de Biologie Structurale (IBS) – UMR5075 (CNRS/CEA/UGA) 71, avenue des martyrs – CS 10090 – 38044 GRENOBLE CEDEX 9, France
http://www.ibs.fr/research/research-groups/pneumococcus-group-t-vernet/
Grenoble is situated in the middle of the beautiful French Alps, and provides a unique environment for state‐of‐the‐art integrated cellular and structural biology (http://www.psb‐ grenoble.eu/).
Contact:
Applications (CV, motivation letter and 2 reference letters) should be sent to Cécile Morlot Email: cecile.morlot@ibs.fr Phone: +33 (0)4 57 42 86 55

Proposé par: Cecile Morlot
Email: cecile.morlot@ibs.fr
Website: http://www.ibs.fr/research/research-groups/pneumococcus-group-t-vernet/
Laboratoire/Institution: Institut de Biologie Structurale
Adresse: 71, avenue des martyrs – CS 10090, Grenoble, 38044, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Post-doc-offer-DIVinHD.pdf

Open post-doctoral position Intravital correlative imaging of tumor metastasis

Type d’annonce: Proposition – PostDoc

Description de la proposition:

The Goetz lab (www.goetzlab.com) is looking for a motivated post-doctoral fellow interested in dissecting tumor metastasis using intravital correlative microscopy. The work will be performed in Strasbourg (France) under the supervision of Jacky G.Goetz, in collaboration with international experts of tumor metastasis and imaging.
Our team studies the molecular and biomechanical events underlying tumor invasion, angiogenesis and metastasis. To this end, we are using a combination of models ranging from in vitro cell biology and biophysical assays (Goetz et al., Cell, 2011) to in vivo animal models of tumor progression (mice and zebrafish) (Goetz et al., Cell Reports, 2014). In particular, we are developing intravital imaging and intravital CLEM (Correlated Light and Electron Microscopy) technologies for tracking subcellular events in vivo at high-resolution (Karreman et al. PloS One 2014; Karreman et al., Journal of Cell Science, 2016; Karreman et al., Trends in Cell Biology, 2016). This development is performed in close collaboration with Y.SCHWAB (EMBL, Heidelberg). More recently, our team was involved in the identification of a new molecular driver of exosome biogenesis (Hyenne et al., Journal of Cell Biology, 2015).
This project aims to dissect the subcellular mechanisms underlying tumor metastasis at very high-resolution using intravital correlative microscopy. Metastasis can be considered as the end product of a multistep process where cancer cells disseminate to distant organs and home in a new tissue microenvironment. Metastases are responsible for the large majority of cancer-related deaths. However, the molecular and cellular mechanisms driving metastasis formation remain to be elucidated and better described in a realistic in vivo context. In collaboration with the team of Y.Schwab (EMBL), we recently developed a technique called intravital correlative microscopy. Here, we propose to apply the newly-developed technology to increase our understanding of tumor metastasis, in particular in terms of cell protrusitivity, proteolytic activity, adaptability to local physical barriers and ability to communicate with its surrounding during the metastasis cascade.
The project involves collaboration with international teams (EMBL, DKFZ, Curie institute) and uses state-of-the-art imaging technologies and animal models. We are looking for an enthusiastic and motivated fellow (who recently defended his Ph.D), with a background in cell and cancer biology and imaging. Expertise in intravital imaging and electron microscopy will be very much appreciated.
Interested candidates should apply as soon as possible or before: 2016 October 1st

Contact:
Jacky G.Goetz, jacky.goetz@inserm.fr, INSERM U1109, Strasbourg, France
Web: www.goetzlab.com, Twitter: @GoetzJacky

Associated references:
1. Karreman, M. A. et al. Correlating Intravital Multi-Photon Microscopy to 3D Electron Microscopy of Invading Tumor Cells Using Anatomical Reference Points. PloS One 9, e114448 (2014).
2. Karreman, M. A. et al. Fast and precise targeting of single tumor cells in vivo by multimodal correlative microscopy. J. Cell Sci. 129, 444–456 (2016).
3. Follain, G., Mercier, L., Osmani, N., Harlepp, S. & Goetz, J. G. Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology. J. Cell Sci. (2016). doi:10.1242/jcs.189001
4. Karreman, M. A., Hyenne, V., Schwab, Y. & Goetz, J. G. Intravital Correlative Microscopy: Imaging Life at the Nanoscale. Trends Cell Biol. (2016). doi:10.1016/j.tcb.2016.07.003

Proposé par: JACKY GOETZ
Email: JACKY.GOETZ@INSERM.FR
Website: http://WWW.GOETZLAB.COM
Laboratoire/Institution: INSERM U1109
Adresse: 3, avenue Molière, Strasbourg, 67200, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Add_MetaCLEM_post-doc-september-16.pdf