Polarbio: Polarimetric orthogonally breaking imaging applied to the study of the dynamics of intracellular architecture

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Interdisciplinary Post-doctoral position in optics and biology in Rennes

The objective of the Polarbio project is to integrate an innovative polarimetric imaging methodology on a conventional confocal microscope within the MRic imaging platform of the University of Rennes 1 in order to produce polarimetric contrast images on different types of biological samples. After a first proof of concept,
two research axes will be investigated: (i) the use of this contrast to characterize biological structures and (ii) the refinement of the microscopy method to improve acquisition speed and data analysis, such as wide field detection or new modes of polarization at the excitation or the emission side.

Research Laboratories: IGDR – R&D Microscopy team /Optics and Photonics dpt. (FOTON Institute)
Heads of the Scientific Project: Marc Tramier / Mehdi Alouini
Contacts: marc.tramier@univ-rennes1.fr / julien.fade@univ-rennes1.fr

Proposé par: Julien Fade
Email: julien.fade@univ-rennes1.fr

Laboratoire/Institution: Institut de Génétique et Développement de Rennes
Adresse: Campus Santé Villejean, RENNES, 35042, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/6/Post-doc-Offer-Polarbio-1.pdf


Type d’annonce: Proposition – PostDoc

Description de la proposition:

We are seeking a highly motivated postdoctoral fellow to study CgA/phosphatidic acid (PA) interaction at
the membrane level in living neuroendocrine cells. To do this, fluorescent molecular tools are available
and novel photoactivatable and biocompatible PA probes are currently synthesized. By combining cell
biology and biochemistry methods, state-of-the-art imaging methods in cellular and in vivo models, the
successful candidate will study CgA/PA interactions and the consecutive formation of microdomains at the
endomembrane level. Therefore, we will test the ability of our chemically synthesized PA analogues to
rescue membrane trafficking defects in neuroendocrine cells from transgenic animal models.
The candidate should have completed a PhD in biochemistry, molecular biology or cell biology. Experience
with membrane dynamics, fluorescence microscopy on live cells and image analysis is required. The
candidate will be working at Inserm 1239, in collaboration with CNRS UPR 3212 (Strasbourg), CNRS UMR
6014 (Rouen) research units and the CIML platform (Marseille) to take benefit of an interdisciplinary
environment (with biologists, biochemists, chemists and physical chemists) and of state-of-the-art
facilities for cell imaging (confocal, TIRF, gated-STED, electron, light sheet microscopies), fluorescence
correlation spectroscopy, flow cytometry, proteomics and animal housing.

Proposé par: Maité MONTERO
Email: maite.montero@univ-rouen.fr

Laboratoire/Institution: Inserm U1239
Adresse: Université de Rouen, Rouen, 76000, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/6/profil-Montero-post-doc-FRM.pdf

Post-doctoral position in Multiphoton and FLIM microscopy for metabolic imaging

Type d’annonce: Proposition – PostDoc

Description de la proposition:

The Advanced Microscopies & Tissue Physiology group at the Laboratory for Optics and Biosciences (LOB), Ecole Polytechnique, University of Paris-Saclay, France, is seeking a highly motivated and talented postdoctoral scientist with interest in interdisciplinary optical microscopy and biophysics research.

The Laboratory develops advanced methods in nonlinear optics, tissue microscopy, image analysis, cell/developmental biology, and biophysics for studying intact biological tissues with subcellular resolution.

Candidates should have a solid background and good experimental skills in optics and/or microscopy and should be strongly motivated and keen to work in an interdisciplinary research field. Previous experience in optical imaging and fluorescence lifetime microscopy will be an asset.

The successful candidate will develop novel approaches for fast fluorescence lifetime imaging of endogenous fluorophores in stem cells and living tissues with application to metabolic imaging (Stringari et. al PNAS 2011, Stringari et al Sci. Rep 2012, Aguilar-Arnal et. al PNAS 2016, Stringari et al Sci. Rep 2017).

The postdoctoral fellowship is funded by a 2017 Human Frontier Science Program Young Investigator collaborative grant. The awarded project: « Chromatin dynamics and nuclear metabolism: an intimate interplay uncovered by non-linear optics » aims to understand epigenetic mechanisms during stem cell differentiation and in particular investigate the influence of cellular metabolism on gene expression.

The postdoctoral appointment will be for 2 years. Detailed information about the research can be requested via email. Interested applicants should submit a CV to Chiara Stringari at chiara.stringari@polytechnique.edu before September 15th. Applications will be reviewed on a rolling basis, starting immediately.

Proposé par: Chiara Stringari
Email: chiara.stringari@polytechnique.edu
Website: https://portail.polytechnique.edu/lob/en/research/advanced-microscopies-tissue-physiology
Adresse: Ecole Polytechnique Route de Saclay, Palaiseau, 91128, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/6/2017-07-Postdoc_FLIM_Stringari.pdf

Adaptive Optics for aberrating and scattering media

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Optical microscopy in biology develops today along two major directions: improving imaging quality, especially in terms of resolution and 3D field of view; and improving content and quantitativeness of associated measurements, such as molecular concentration and dynamics (diffusion, interactions, etc.). For obtaining the latter information, a very successful and well-established technique is Fluorescence Fluctuation Microscopy (FFM), which is a generalisation of classical Fluorescence Correlation Spectroscopy (FCS), which provides quantitative measurements of molecular concentration and mobility in living specimen, by analyzing the signal fluctuations caused by fluorescent molecules as they diffuse across a small observation volume. Within this context, the main goals of the μ-SCATTAB project are: i) to understand how light scattering and optical aberrations disturb measurements performed with Fluorescence Fluctuation Microscopy; ii) to develop new schemes to correct for these effects; iii) or alternatively, to take benefit of light scattering to perform molecular measurements.
In the past, Adaptive Optics (AO) approaches have been successfully used in microscopy for correcting sample-induced aberrations, but the majority of these methods have been concerned with compensating low-order aberrations which arise in rather transparent samples or at shallow focusing depth. However, when focusing deep into a specimen (tens to hundreds of μm), large-amplitude aberrations with complex phase structure arise, and light scattering dramatically increases. In our project, we will investigate how to take these effects into account and what are the most efficient correction schemes and strategies for FFM. Complementary experiments and numerical / theoretical calculations will be conducted by the Grenoble and Göttingen partners, which will imply visits between the two groups. We are looking of a post-doctoral researcher to join the Grenoble group: the contract should begin in September or October 2017 for a total duration up to 30 months.
The successful candidate would have a background in optics with strong experience in instrumental optics/microscopy. Previous experience in wavefront manipulation, adaptive optics or imaging through scattering media would also be highly appreciated. The recruited post-doc will join an interdisciplinary team of physicists and biophysicists working on living systems at the single cell or multicellular scale. He/she would be in charge of modifying a currently existing adaptive confocal microscope available in Grenoble to add a wavefront corrector with a large number of degrees of freedom and developing new strategies to perform FFM measurements in complex media.

Proposé par: Antoine DELON
Email: antoine.delon@univ-grenoble-alpes.fr
Website: http://www-liphy.ujf-grenoble.fr/Equipe-MOTIV
Laboratoire/Institution: laboratoire Interdisciplinaire de Physique (LIPhy)
Adresse: Université Grenoble Alpes Pôle Phitem Laboratoire LIPhy CS 40 700, GRENOBLE CEDEX 9, 38058, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/µ-scattab-position-final.pdf

Postdoctoral Position – Zebrafish models of proteinopathies in neurodegenerative diseases– Institut de Recherches Servier

Type d’annonce: Proposition – PostDoc

Description de la proposition:

The Servier’s Neuropsychiatry Innovation Therapeutic Unit accommodates a zebrafish platform as a part of its experimental research, located near Paris, in Croissy-sur-Seine, France. The platform is involved in early drug development and is strongly oriented towards providing tools to screen compounds intended for neurodegenerative diseases. The zebrafish team is also involved in the project IMPRiND, which is a public-private partnership, supported by the Innovative Medicines Initiative (IMI) and will officially start on Q2 2017. The project’s goal is to identify disease-relevant misfolded assemblies of alpha-synuclein and tau proteins and validate complex cellular systems and improve existing animal models of Alzheimer’s and Parkinson’s disease.
We are looking for a Postdoctoral research Associate to contribute to the development and characterization of zebrafish models for alpha-synuclein and tau propagation in conjunction with the project IMPRiND as well as our internal research activities. The ideal candidate will have a PhD in biological sciences or related disciplines, with a previous experience in the field of proteinopathies, strong knowledge of immunostaining, fluorescence microscopy and image analyses, as well as a strong interest in using zebrafish as a tool for drug discovery and target validation in neurodegenerative diseases. Good communication skills in English and French are a must to interact with the partners in the European consortium IMPRiND and the multi-disciplinary team at our Research Center, respectively.
Servier is an independent research-based pharmaceutical company headquartered in France. With a strong international presence in 148 countries and 92% of its medicines being prescribed outside of France, Servier employs more than 21,200 people worldwide. In 2015, the company recorded a turnover of 3.9 billion euros of which 24% was reinvested in research and development.

Proposé par: Anne Dekeyne
Email: anne.dekeyne@servier.com

Laboratoire/Institution: Institut de Recherches SERVIER
Adresse: 125 chemin de ronde, Croissy sur Seine, 78720, France
Details en pdf: