Modeling the mechanisms of cytoskeleton reorganization in platelets

Type d’annonce: Proposition – PostDoc

Description de la proposition:

The project concerns the mechanisms of cytoskeleton reorganizations and crosstalks that drive blood platelet activation. Its precise regulation ensures hemostasis and prevents thrombosis. The flat shaped disc formed by quiescent platelets is maintained by the marginal band (MB), a peripheral ring of microtubules. We have shown recently that the initial shape change during platelet activation is due to cytoskeleton (microtubules and actin) and motor­driven MB extension inducing MB’s coiling and consequently its change from a disc to a spherical shape (see the figure below). The postdoctoral fellow will develop a biomechanical multi­scale and individual­based model of microtubules, actin filaments and motors to understand the manner the MB forms and reorganizes as the levels of platelet activation change (due to microtubule acetylation, molecular motor expression levels, …).

Proposé par: arnaud chauviere
Email: arnaud.chauviere@imag.fr
Website: http://www-timc.imag.fr/
Laboratoire/Institution: TIMC-IMAG / UGA
Adresse: Pavillon Taillefer, domaine de la Merci, La Tronche, 38706, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Postdoctoral_position_BiologicalModelling.pdf

développement d’une source OPA pour de la microscopie non-linéaire

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Le Laboratoire Charles Fabry (LCF) à l’Institut d’Optique de Palaiseau recrute un post-doctorant dans le cadre d’une collaboration entre le LCF et le Laboratoire d’Optique et Biosciences (LOB) de Polytechnique. Ce post-doctorat s’intègre dans les projets Morphoscope (Equipex) et DeepIM du Labex PALM. La durée du contrat est de 2 ans, à pourvoir dès que possible.
Le projet vise à développer un instrument d’imagerie innovant pour effectuer de la microscopie non linéaire rapide et en profondeur. Cette nouvelle microscopie repose, tout d’abord, sur l’utilisation d’une source laser femtoseconde, haute-cadence, originale -à base de fibres-, convertie ensuite par amplification paramétrique optique (OPA) dans la bande du moyen-infrarouge (MIR). L’utilisation d’impulsions MIR, au lieu de sources classiques émettant dans le visible ou le proche infrarouge, permet de diminuer l’impact de la diffusion et d’obtenir des images au faîte de l’état de l’art en termes de profondeur.
Le ou la post-doctorant(e) participera donc au développement de la source dans le moyen infrarouge et à son implémentation sur un bâti de microscope afin de réaliser des images en profondeur de tissus biologiques. Il ou elle devra donc être motivé(e) par ces thématiques à l’interface entre les lasers et la biologie. Des compétences en instrumentations optiques, systèmes femtoseconde, amplification paramétrique optique, microscopie non-linéaire seront grandement appréciées. En effet, le ou la candidat(e) sera amené(e) à développer un projet de recherche sur l’amplification paramétrique optique dans le MIR pour des applications en microscopie.
Le développement d’un tel outil dédié à la microscopie est une première scientifique et s’inscrit clairement dans une thématique émergente de la recherche à l’interface laser/bio-imagerie. Cet instrument unique combinant sources laser à l’état de l’art et nouvelles techniques de microscopie non-linéaire montre un très grand potentiel pour réaliser des percées importantes dans le domaine de la bio-imagerie de tissus vivants.

Proposé par: Frédéric Druon
Email: frederic.druon@institutoptique.fr

Laboratoire/Institution: Laboratoire Charles Fabry
Adresse: 2 av. Augustin Fresnel, Palaiseau, 91127, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/post-doc-offer-morphoscope-fr-en.pdf

Application for postdoc/engineering position_Bordeaux

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Team: Quantitative Imaging of the Cell
Type of contract: 12 months, renewable, full time
M.S/PhD in a computer science, bioinformatics or biotechnology
Salary: commensurate with experience
Characteristic duties
We are searching a motivated engineer/postdoc to create and expand on existing software solutions dedicated to biologists’ needs for the acquisition and analysis of High Content Screening (HCS) experiments on super-resolution optical microscopes.
Under the responsibility of the project leader and in close collaboration with the biology and bioinformatics teams, he/she will:
– Develop the software and graphical user interface for the acquisition (screening) and create a database from super-resolution microscopic image analysis (localizations, tracking, …)
– Implement new classification methods and evaluate them on biological data.
Requirements
– Language: Visual Basic .NET, C/C++. Optional: Python, Matlab, Java, R.
– Database IT, SQL.
– Knowledge of fundamentals of microscopy (Experience with Metamorph is a plus).
– Excellent communication skills, openness, and ability to collaborate in an interdisciplinary environment.
The team
The “Quantitative Imaging of the Cell” team develops novel imaging techniques to better understand the living cell activity at high spatial and temporal resolutions, in a high throughput context. The team works in close collaboration with the biology teams of IINS, the Bordeaux Bioinformatics Center (CBiB) and some industrial partners
Contact
Jean-Baptiste Sibarita: jean-baptiste.sibarita@u-bordeaux2.fr

Proposé par: Anne BEGHIN
Email: anne.beghin@u-bordeaux.fr
Website: http://www.iins.u-bordeaux2.fr/
Laboratoire/Institution: IINS CNRS UMR 5297
Adresse: Bât PGF Université Bordeaux 2 146 rue Léo Saignat, Bordeaux, 33077, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Offre-de-poste-info-IINS_englishpostdoc1.pdf

How is coordinated DNA damage repair and control of mitotic commitment ensuring long-term genomic stability during successive cell cycles?

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Post-doctotal applicant

Proposé par: Olivier GAVET
Email: olivier.gavet@gustaveroussy.fr
Website: http://gustaveroussy.fr/fr/content/division-cellulaire-et-stabilité-génomique-thématique
Laboratoire/Institution: Institut Gustave Roussy
Adresse: UMR8200 CNRS Pavillon de recherche 2, 114 rue Edouard Vaillant, Villejuif, 94805, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Postdoc-position-2015.pdf

Offre de postdoc: Micsroscopy and FACS screen for asymmetric segregation of chromatin components in S. cerevisiae

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Asymmetric cell division is a prerequisite for cellular differentiation and stem cell maintenance. Phenotypic transformation during differentiation is a poorly understood epigenetic phenomenon, in which chromatin, as a transcriptional regulator, theoretically plays a role. The underlying assumption that chromatin components segregate asymmetrically in asymmetric divisions has however not been systematically tested. Budding yeast also undergoes asymmetric divisions producing mother and daughter cells. The mother can generate 30-40 daughters during its replicative lifespan, and most of the factors that determine the phenotypic identity of the mother cell are unknown. The high conservation of chromatin components among eukaryotes and the availability of powerful genetic tools, make S.Cerevisiae an ideal system for identifying chromatin components involved in asymmetric division and elucidating the underlying molecular mechanisms, using whole genome and single cell approaches.
The two year position is financed by the ANR. The candidate will implement a screen for asymmetric segregation of chromatine components between the mother and the daughter cells. He/She will perform live cell fluorescence microscopy (in combination with a microfluidic device later in the project) and FACS analysis of yeast strains containing proteins of interest fused to photoswitchable fluorescent reporters.
Candidates with experience in live cell fluorescence microscopy and yeast biology will be considered first.

Proposé par: Marta Radman-Livaja
Email: marta.radman-livaja@igmm.cnrs.fr
Website: http://www.igmm.cnrs.fr/spip.php?rubrique159
Laboratoire/Institution: IGMM/CNRS
Adresse: 1919 route de Mende, Montpellier, 34293, France
Details en pdf: