Toward nonlinear Multimodal Endomicroscopy combining large field of view and high resolution imaging

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Current surgical biopsy takes several days to be analyzed by anatomopathologists, who provide analysis report to the surgeon a few days after the surgical intervention. Moreover, tissue biopsies are often associated with patient discomfort as they are highly invasive. In addition, the lack of precise guidance often leads to inaccuracies in the selection of tissue regions for biopsy. Eliminating this time consuming process of conventional biopsy is a clinical need, as well as increasing the accuracy tissue diagnostics and the patient comfort. We propose to address these needs by developing a nonlinear endomicroscope able to give optical biopsy results on the spot, which will lead to the immediate diagnosis and an orientation of the choice for optimal therapy means. It will also limit the extraction of tissue sample for conventional biopsy analysis. In this instrument we will combine different imaging contrasts, including nonlinear optical fluorescence imaging (TPF), second harmonic generation (SHG), reflectance, lifetime and spectroscopic analysis. This multimodality is crucial to allow a reliable analysis of optical signal by anatomopathologist in comparison to the gold standard Hematoxilin and Eosin histological analysis. Fibered endomicroscope could be used during the surgical operation thus offering deep subcellular resolution without adding invasivity.
The recruited post-doc will be involved exclusively in the optical part of the project. It will be focalized on the study of the choice of the adapted miniaturized optics to the specific microstructured fiber. He/She will participate to the assembling and characterization of a customized DCF with this optics. He/She will be in charge to validate of the global collection architecture of the endomicroscope systems in term of efficiency and multimodality. In parallel, he/she will be involved in scanning system assembly.
The project is funded by the PlanCancer and is in collaboration with XLIM laboratory and Sainte Anne Hospital. The position can start in February 1st 2015 or later, and will be located at IMNC lab, Orsay, France.
Required skills:
– PhD in physics or medical imaging.
– Strong experience in optical instrumentation and optical fiber.
– Knowledge in fluorescence tissue imaging is welcome.
– Skill of Matlab, labview and Zemax for optical design will be appreciated
Duration:
– 1 year
– A possibility for a prolongation of 6 months
Contact:
Please send your candidature (CV + letter of motivation + 2 contact persons) to:
Darine ABI HAIDAR (abihaidar@imnc.in2p3.fr)

Proposé par: darine abi haidar
Email: abihaidar@imnc.in2p3.fr

Laboratoire/Institution: IMNC
Adresse: Campus universitaire – bât 440, orsay, 91405, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Postdoctoral-position-D.pdf

POST-DOC GRENOBLE Liphy

Titre :  Unraveling the physics of cancer growth in confined environments

Type d’annonce: Proposition – PostDoc

Description de la proposition:

The growth of solid tumors occurs in constrained environments. The expanding neoplastic tissue compresses the surrounding tissues and thus builds up and stores an internal stress. Little is known about the mechanical reaction of the tumor to this stress.
The reason is that the tumor escapes to a univocal classification, in terms of mechanics. If fact, it responds as a viscoelastic material, but actively changes its stiffness and size when mechanically stressed.
In addition, cells collectively flow inside the tumor.
The aim of this project is to track the cells position inside a growing tumor in 3D and in real time. Based on the experimental observations, we want to build up a theoretical description of the tumor as an “active material”.
Thus, we will propose a predictive model to describe the cellular motion inside the tumor tissue under compression.
The candidate will observe in real time the local cellular organization inside a multicellular spheroid and determine how it is affected by cell division and death. She/He will also measure and model the variation of cell volume after exposure to the compression, as a function their position inside the spheroid.
The project is supported by an INSERM contract with a two years postdoctoral grant. It will take place in a transdisciplinary team gathering physicists, biologists and opticians and it will be developed in collaboration with the Institut Curie and the ESPCI (Paris). The applicant should have a good background and training in biophysics. Experience in data analysis, simulation, microfluidics and cell culture could be appreciated.

Proposé par: Antoine Delon
Email: Antoine.Delon@ujf-grenoble.fr

Laboratoire/Institution: Laboratoire Interdisciplinaire de Physique,Materiaux, Optique et Techniques Instrumentales pour le Vivant
Adresse: 140 Av. de la physique – BP 87, Saint Martin d’Hères, 38402, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/Postdoctoral-Position1.pdf