The program of the 2016 session of the thematic school Mifobio includes the following course modules, including 2 courses during the plenary session and 2×2 courses organized in two parallel sessions on two different themes. For each module are given the names of speakers who have confirmed their attendance.
The courses have educational content to put into perspective the fundamentals and technical developments. The seminars help to showcase the latest results of some dedicated laboratories. Practical workshops address those subject directly manipulating on microscopes (imaging) or computers (image analysis).
• Seminars :
- Alberto Diaspro, university of Genovia, Italy
- Jan Ellenberg, EMBL, Heidelberg, Germany
- Paul French, Imperial College, Londres, UK
- Benny Geiger, Weizmann institute of Science, Rehovat, Israel
- Antoine Triller, École Normale Supérieure, Paris
- Eric Röttinger, INstitut for research on cancer and aging, Nice
- Ivo F. Sbalzarini, Center for Systems Biology, Dresden, Germany
- Benoit Weil, Mines Paris Tech, Paris
• Nanoscopic cell organization and correlative microscopy
Coordinator(s) : Sandrine Lévèque-Fort, Jean-Baptiste Sibarita
This module focuses on the concept of “super-resolution” (SIM, PALM/STORM, STED, etc.) and correlative (Fluo+EM, Fluo+probe) imaging techniques, enabling the observation of living cells at the structural and molecular level. Super-resolution techniques rely on the chemical and photo-physical properties of fluorescent molecules, the way they are excited, and the computerised processing and analysis of the resulting images. Correlative microscopies open a novel path to observe the cell’s ultrastructure by combining the advantages of optical and electron microscopies, raising challenges in sample preparation and data alignment across modalities.
L. Wesley, Janelia Research campus, USA
E. Lemke, EMBL, Heidelberg, Allemagne
J.B. Sibarita, Institut interdisciplinaire de Neurosciences, Bordeaux,
A. Fragola, Laboratoire Physique et études des matériaux, ESPCI, Paris.
M. Heilemann, Institute of Physical and Theoretical Chemistry, Francfort, Allemagne
J. Chojnacki, Weatherall Institute of Molecular Medicine , University of Oxford, UK
Coordinator(s) : Alexandre Dufour, Martial Balland, Karine Anselme
The cell is a complex molecular super-structure that naturally undergoes physical environmental constraints to which it must adapt & respond to, notably in situations of stress. Cells exhibit great mechanical and functional plasticity at numerous levels (from the outer membrane down to the chromatin). They are also able to organise in groups and communicate within tissue and organs, thereby gaining new mechanical properties, thus reaching a remarkable level of functional complexity. Studying cellular and tissular biomechanics is of particular interest for both biologists and physicists, who develop a wide range of interdisciplinary techniques to understand their dynamics.
A.Roux, Departement de biochimie, Genève
K.Wolf, Department of Cell Biology, Radboud University Nijmegen Pays Bas
N.Gauthier, Mechano Biology Institute, Singapour
R.Paterski, Departement de biochimie, Université de Zurich, Suisse
A.Boudaoud, ENS Lyon,
C.Allier/L.Hervé, CEA, Grenoble
• Neuroscience Imaging : Interdisciplinary challenge
Coordinator(s) : Laurent Bourdieu & Lydia Danglot
Imaging technologies have largely contributed to scientific advances in neurosciences over the last decades, notably for the study of the synapse via super-resolution approaches and cerebral activity using functional imaging. The aim of this module is to illustrate the recent advances in the field made possible by an interdisciplinary community, and how the futur challenges in neuro-imaging are anticipated.
D.Choquet, Institut des neurosciences, Bordeaux
D. Peterka, Columbia Univ, New York
T. Ryan, Cornell Univ, New York
A.Packer, University College of London, Londres, UK
C.Ventalon, Ecole Normale Supérieure, Paris
G. Debrégeas, Pierre et Marie Curie Univ, Paris.
• Photomanipulation, optogenetic and biosensor
Coordinator(s) : Mathieu Coppey
The recent development of techniques enabling the spatio-temporal control and modulation of gene function permit the understanding of various complex processes of life (notably in neurobiology and development). These techniques are in perpetual evolution, requiring both the development of novel techniques as well as new modelling tools.
O. Griesbeck, Institut Max Planck de neurobiologie, Munich
B.Di Ventura, EILSLABS, Université de Heidelberg, Allemagne
H.Mizuno, Departement de chimie, KU Louvain
O.Destaing, Institut Albert Bonniot, Grenoble
A.Gautier, Ecole Normale Supérieure, Paris
P.Vincent, IBPS, Paris
• Molecular assembling and dynamics: from experimentation to modeling
Coordinator(s) : Hugues Berry, Laurent Héliot, Cyril Favard
The study of cellular mechanisms, their regulation and integration, requires the measurement of the dynamics of molecular interactions within live cells, be this at the single molecule level or at a larger statistical scale via spectroscopy techniques. Results from both sets of approaches are complementary, but hard to combine. Tight interaction between experimentalists and theoreticians enable new advances in this field, opening the way towards an integrative view of elementary molecular processes, while guiding further experimental developments.
T. Wohland, Université de Singapour
F.Nedelec, EMBL, Heidelberg, Allemagne
M. Digman, Université de Californie, USA
P.Wiseman, McGill university, Montreal, Canada
A.Coulon , Institut Curie, Paris
I. Izeddin, ESPCI, Paris
• Imaging in tissues, challenge of diffusion and heterogeneous environments – Adaptive Optics
Coordinator(s) : Sophie Brasselet
This module is organised, as in the 2014 edition, in collaboration with the “GdR Ondes”. Its goal is to extend the realm of live imaging techniques to other modalities (ultrasound, etc.), but also to bring novel concepts such as the spatio-temporal waveform control (imaging through diffusing tissue).
P.Beard, UCL, Londres, UK
M.Booth, Dept of Engineering Science, Université d’Oxford, UK
F.Palambo, Université d’ Exeter, UK
E.Papagiakoumou, Université Paris Descartes, Paris
O.Katz, Jerusalem, Israel
O.Couture, Institut Langevin, ESPCI, Paris
• Multi-scale imaging and integrative biology
C. Xu, Cornell University, USA
S. Preibisch, Berlin Institute of Medical Systems Biology, Berlin
F. Louradour , Xlim, Limoges
N. Dostatni, Institut Curie, Paris
R. Galland, Institut des neurosciences, Bordeaux
W. Suppatto, LOB, École Polytechnique, Palaiseau