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MIFOBIO 2014

Rationale

The study of major cellular functions and their integration at the tissue and organism levels is currently limited by several technological/methodological barriers. In order to overcome these obstacles, it is necessary to bring together expertise from fields such as Chemistry, Physics, Mathematics, Biology and Computer Sciences. Imaging is mandatory in this context, especially to develop new strategies in multimodal, analytical and quantitative microscopy for the different complexity levels of biology. While expert labs in the imaging field may develop accurate solutions, it is also important to make them accessible to a broad community of researchers. In this respect, imaging core facilities are ideally positioned to adapt and transfer innovative technologies to their perimeter, if their expert staff keeps going with know-how and knowledge acquisition.
To connect all partners in the functional microscopy field and to allow the emergence/birth of a think tank in this biotech field, the GDR2588 and the « réseau technologique des microscopes photoniques » organize since 2004 (every two years), in close collaboration with the CNRS educational training office, this thematic school entitled: functional microscopy in biology (MiFoBio).

Pratical informations

MiFoBio 2014 summer school will be held from the 4th to the 10th of October 2014 at the « Belambra Le Tuquet » in Seignosse (Landes).
Bus shuttles will be rotating between the TGV train station or airport of Biaritz on October 4rd and 10th .

Interests/Objectives

Context

The study of the main cell functions, such as gene expression, membrane trafficking, cell signaling as well as cell mobility or their organization in tissues and small organisms requires the analysis of many entities interactions and dynamics. The localization of these molecular events in isolated cells and living organisms, their measurement and quantification are essentially based on new developments in microscopy.
These developments are so far limited by technological constraints (probes, targeting, detection, seinsitivity, phototoxicity, image analysis, modelling). Overcoming this, requires

  1. Bringing together skills from various scientific domains such as chemistry, physics, computer sciences, mathematics and biology,
  2. The development of new strategies in analytical and quantitative microscopy (biological, technological and cognitive solutions),
  3. The use of emerging techniques such as femtosecond lasers, time resolved fluorescence measurement,
  4. Computer tools for image analysis, modeling and data management.

The overall aim of MiFoBIO is to create the best synergy in order to fulfill these necessities. The immediate goal is to organize the best conditions in terms of knowledge sharing, skill learning, and networking

Aim :

The aim of this summer school will be the development of functional studies of cells and integrated cellular systems by bringing together experts and specialists from various scientific disciplines that will ultimately share their knowledge and know-how. This school thus aims at increasing the interaction, the interactivity and brainstorming capacity between scientists from various domains, such as : chemistry, biochemistry, physics, instrumentation, cell and developmental biology, image analysis, applied mathematics …
This will be done

  1. By giving the conceptual and practical basis in the different domains involved.
  2. By searching solutions to solve problems due to the technical challenges of biology
  3. By defining new methodological solutions for particular biological questions.
  4. By developing interactions between laboratories from different background.

Typical attendees:
Core facility Staff or not, academic and industry researchers, engineers, post-doctoral fellow or PhD student pertaining to a field involved in the development or the use of functional microscopy. As for the last two Mifobio sessions (2010, 2012) we apologize to have to restrict the number of participants to 350 people.

Overall organization and Program

The program is best introduced by courses aimed at giving short basic knowledge to all participants, whatever their scientific background.

Starting-modules

  1. Cell organisation and study methods
  2. Principle of optics for microscopy
  3. Introduction to image analysis
  4. Bases of Fourrier Transform for imaging

The overall program is organized into defined modules that are changing every Mifobio sessions, depending on the scientific achievements and actualities in the field. Those modules are constituted of regular courses (from more or less basic to advanced levels, given by French and foreigner specialists) and intensive related practical workshops with real “hands-on” sessions, proposed by academic labs and most often co-organized with our industrial partners. The program is completed by series of 15 round tables, 1 large poster session and 4 to 6 conferences-seminars of general interest, given by invited speakers.

Modules

Module 1 : Molecular organization and nanoscopy

Suliana Manley, EPFL, Suisse
Laurent Groc, Bordeaux neurocampus, France
Gael Moneron, Institut Pasteur, Paris, France
Lothar Schermelleh, Univ. Oxford, Grande-Bretagne
Mike Heilemann, Francfort, Allemagne,
Maxime Dahan, Paris, France

Module 2 : Mecano-biology in cells and tissues: experimentation and modelling

Klemens Rottner, Bonn, Allemagne
Stefan Grill, Dresden, Allemagne
Laetitia Kurzawa, Grenoble, France
Olivier Rossier, Bordeaux, France
Benoit Ladoux ou René-Marc Mège, Paris, France
Martial Balland, Grenoble, France

Module 3: Multi-scale imaging for biology from molecules to organims

Yohanns Bellaïche, Paris, France
Alex McDougall, Villefranche sur Mer, France
Julien Vermot, Illkirch, France
Emmanuel Farge, Paris, France
Willy Suppato, Palaiseau, France
Nadine Peyriéras, Gif, France

Module 4 : Optogenetic, biosensors and photo-manipulation

Kees Jalink,  Amsterdam, Pays-Bas
Ludovic Jullien, Paris, France
Christoph Gebhart, Paris, France
Mathieu Coppey, Paris, France
May Morris, Montpellier, France

Module 5: Dynamics and molecular interactions in live cells

Paul Wiseman, Montréal, Canada
Amer Beg, Grande Bretagne
Jorg Langowski, Allemagne
Emmanuel Margeat, Montpellier, France
David Holcman, Paris, France
Hugues Berry, Lyon, France

Module 6: : wavefront engineering for bio-imaging

Delphine Débarre, Grenoble, France
Emmanuel Bossy, Paris, France
Sylvain Gigan, Paris, France
Stefan Catheline, Lyon, France
Hervé Rigneault, Marseille, France
Bertrand Audoin, Bordeaux, France

Module 7: new imaging, multi-modal and correlative microscopies

Andreas Zumbush, Constance, Allemagne
Yannick Schwab, Heidelberg, Allemagne
Sophie Brasselet, Marseille, France
Christel Genoud, Bâle, Suisse
Grégoire  Malandain, Sophia Antipolis, France
Frank Lafont, Lille, France

Workshops

We have an outstanding technology park (unique in Europe) thanks to the participation of industrial partners and to academic labs which bring their prototypes. In total, more than 70 workshops will be conducted by teachers and expert attendees on this equipment. The goal of these workshops is to put in practice the content of the lectures and to make biologists, physicists, chemists, computer scientists,… working together.
Two computer rooms are available for image processing and analysis workshops
Two kinds of workshops will be implemented: Applicative and experimental workshops (the latter for the evaluation of new experimental approaches)
According to the schedule and number of attendees each workshop will occur once or twice. Workshop sessions are typically scheduled for about 2 hours, except for the ones at night which can last very late, sometimes.
Diverse living cell and small organism models are supplied by workshop organizers and/or provided during the whole School. Culture room, aquarium…facilities are available (thank’s to the “Mifobio culture room” -Team)

15 round tables will be held (1h to 1h30 per round table). They will take place in parallel to the workshops. The topics of the roundtables will be finalized late, yet prior the beginning of the school, in order to stay reactive towards scientific news and /or breakthrough and accommodate participants requests.

Biological models

In order to conduct workshops on live cell samples we will set up a cell culture room with the financial support of our industrial partners.
Similar to previous years, we will host workshops on animal models.

Models available:
-Diverse cell lines to study cytoskeleton, membranes, nuclear organization, signaling pathways…
– Zebrafish
– C.elegans
– Drosophila
– …

Costs

Cost includes training, accommodation and meals on the evening of October 4rd up to October 10th , noon.
– Academic: 970 €