Frontiers of silencing at super resolution

Type d’annonce: Proposition – PostDoc

Description de la proposition:

Laboratory: Nuclear dynamics, UMR 3664
Angela Taddei Team: Compartmentalization and dynamics of nuclear functions.
Starting date: as soon as possible

SUMMARY OF LAB’S INTERESTS:
The eukaryotic genome is packaged into large-scale chromatin structures that occupy distinct domains in the nucleus. This DNA organization is a key contributor to genome functions. Our aim is to understand what determines the spatial and temporal behavior of chromatin and how this affects two essential functions of the genome: gene expression and the maintenance of genome integrity. To understand these fundamental processes, we combine genetics, molecular biology and advanced live cells imaging.

PROJECT:
One of the most intriguing features of nuclear organization is the existence of sub-nuclear compartments enriched with specific DNA, RNA sequences and proteins. How such membrane-less micro-environments are formed and maintained remains unclear. Silent chromatin provides an evolutionary conserved example of such functional sub-compartments. In Saccharomyces cerevisiae yeast, the 32 telomeres of haploid cells cluster into 3-5 sub-nuclear compartments or telomere foci concentrating the SIR complex at the nuclear periphery. Our team has recently shown that this organization is dynamics and varies according to the metabolic status of the cell. In particular, cells group their telomeres into a unique cluster (hypercluster) upon progressive carbon source exhaustion, reshaping the genome architecture into a conformation that may help to maintain the longevity of quiescent cells.

In this project, we propose to combine yeast genetics with super resolution (PALM/STORM) and single particle tracking to decipher the biophysical mechanisms underlying telomere foci formation and dynamics.

We offer a 18-months founded post-doc contract and will support the candidate to apply for post-doc grants.

Key publications:
Multi-scale tracking reveals scale-dependent chromatin dynamics after DNA damage. Miné-Hattab et al., Mol Biol Cell. 2017.
Spatial reorganization of telomeres in long-lived quiescent cells. Guidi et al. Genome Biol. 2015.
Spatial telomere organization and clustering in yeast Saccharomyces cerevisias nucleus is generated by random dynamics of aggreagation-dissciation. Hozé et al., Mol. Biol. Cell. 2013.

ENVIRONMENT:
The Taddei team provides all equipment’s necessary for yeast cells culture and has a strong expertise in field of silencing and quiescence. A super resolution microscope is already available in the unit (PALM/STORM and single particle tracking in 3D with multi colors). Many yeast strains have been already developed for PALM/STORM and single particle experiments. The UMR 3664 of the Curie Institute is a very stimulating environment to work in the epigenetics and DNA repair field. The project is carried out in collaboration with Maxime Dahan’s team (Physico-Chimie Curie, UMR168).

PROFILE AND EXPERTISE:
We invite applications from highly motivated and dynamics individuals holding, or shortly expecting to be awarded, a PhD degree in biophysics. Proven research skills (publication track) as well as good communication skills (in English) are essential.

Expertise:
– Microscopy, image analysis (ImageJ, Matlab)
– Strong interest in cell biology and DNA repair mechanisms
– Knowledge in transcription and genetics is appreciated
– Organization, responsibility and autonomy are required
– An experience in budding yeast and cell culture is appreciated

To apply, please send a letter, your CV including publication record
and contact details for 2 referees at:
Angela.taddei@curie.fr, Judith.mine@curie.fr

Proposé par: Angela Taddei
Email: judith.Mine@curie.fr
Website: https://science.curie.fr/recherche/developpement-cancer-genetique-epigenetique/umr-3664-dynamique-du-noyau/equipe-taddei/
Laboratoire/Institution: UMR3664 Nuclear dynamics, Taddei team / Institut Curie
Adresse: 26 rue d’ULM, Institut Curie Reserche center, Paris, 75005, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/6/Post_Doc_Institut-Curie_Silencing_Super_Resolution_Microscopy-1.pdf