Tomographic Diffractive Microscopy: Development and Applications

Type d’annonce: Proposition – Thèses

Description de la proposition:

Optical microscopy techniques are among the preferred methods for biological studies thanks to their unique capability of imaging living specimens in 3-D. Tomographic diffractive microscopy (TDM) is a new technique [1], combining microholography with tomographic acquisitions. The MIPS laboratory develops this technique in transmission [2-5], in reflection [6,7], and with specimen rotation [8,9].

Our research project now aims at:
– further improving the resolution, in order to obtain an isotropic 3-D resolution in the 100 nm range for non-fluorescent specimens,
– developing new tomographic reconstruction methods, to expand the domain of applicability of TDM (Rytov approximation, complex field deconvolution)
– testing the instrument for biological investigations.
The selected candidate will join an active and recognized research group. Competences in optics/photonics, and/or image processing/instrumentation/computer sciences are necessary.
First, the high-resolution tomographic systems at MIPS will be upgraded. Theoretical and experimental work will be carried out in-home, to test new reconstruction methods. Work on biological samples will be performed through collaboration with IGBMC in Strasbourg (Dr Marc Ruff’s team), with help of a post-doc student to be recruited on the same topic.
Standard Fellowship: 1 684,93 €/month.
For more information, please contact:
Pr. Olivier Haeberlé
Laboratoire MIPS, 61 rue Albert Camus 68093 Mulhouse cedex
Tél: + 33 (0)3 89 33 76 11 Fax : + 33 (0)3 89 33 76 05 olivier.haeberle@uha.fr
[1] O. Haeberlé, K. Belkebir, H. Giovaninni and A. Santenac J. Mod. Opt. 57, p. 686 (2010)
[2] M. Debailleul, B. Simon, V. Georges, V. Lauer, O. Haeberlé, Meas. Sci. Technol. 19, 074009 (2008)
[3] B. Simon, M. Debailleul, V. Georges, V. Lauer, O. Haeberlé, Eur. Phys. J. Appl. Phys. 44, p. 29 (2008)
[4] M. Debailleul, V. Georges, B. Simon, R. Morin and O. Haeberlé, Opt. Lett. 34, p. 79 (2009)
[5] B. Simon, M. Debailleul, A. Beghin, Y. Tourneur, O. Haeberlé, J. Biophotonics, 3, p. 462 (2010)
[6] M. Sarmis, et al., J. Mod. Opt. 57, p. 740 (2010)
[7] H. Liu, J. Bailleul, B. Simon, M. Debailleul, B. Colicchio and O. Haeberlé, Appl. Opt. 53, p. 748 (2014)
[8] S. Vertu, J.-J. Delaunay, I. Yamada and O. Haeberlé, Centr. Eur. J. of Phys. 7, p. 22 (2009)
[9] S. Vertu, J. Flügge, J.-J. Delaunay, and O. Haeberlé, Centr. Eur. J. of Phys. 9, pp. 969-974 (2011)

Proposé par: Olivier Haeberlé
Email: olivier.haeberle@uha.fr
Website: http://www.mips.uha.fr
Laboratoire/Institution: Laboratoire MIPS – Université de Haute-Alsace, Mulhouse
Adresse: 61 rue Albert Camus, Mulhouse, 68093, France
Details en pdf: http://gdr-miv.fr/gdr/wp-content/uploads/formidable/PhDTomoBio2015.pdf